Mutation rate from asparagine requirement to asparagine non-requirement
نویسندگان
چکیده
منابع مشابه
The rate of mutation of L5178Y asparagine-dependent mouse leukemia cells to asparagine independence and its biological consequences.
LSI78Y mouse lymphoblastic leukemia cells normally require asparagine (ASN) for growth, but phenotypically stable ASN-independent cells are detectable at low fre quency by direct cloning, in ASN-free soft-agar medium, of cells from cultures that have been grown nonselectively in medium containing ASN. The rate of mutation in vitro to ASN independence was determined by the Luria-Delbruck fluctua...
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The proteins of Plasmodium, the malaria parasite, are strikingly rich in asparagine. Plasmodium depends primarily on host haemoglobin degradation for amino acids and has a rudimentary pathway for amino acid biosynthesis, but retains a gene encoding asparagine synthetase (AS). Here we show that deletion of AS in Plasmodium berghei (Pb) delays the asexual- and liver-stage development with substan...
متن کاملThe Rate of Mutation of L5178Y Asparagine-dependent Mouse Leukemia Cells to Asparagine Independence and Its Biological Consequences1
LSI78Y mouse lymphoblastic leukemia cells normally require asparagine (ASN) for growth, but phenotypically stable ASN-independent cells are detectable at low fre quency by direct cloning, in ASN-free soft-agar medium, of cells from cultures that have been grown nonselectively in medium containing ASN. The rate of mutation in vitro to ASN independence was determined by the Luria-Delbruck fluctua...
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L-Asparagine is responsible for a major portion of the effect of peptone on the growth of L5178Y lymphoblasts in culture and is essential for the reproduction of these cells. Among the compounds tested, the requirement for the amide is replaced only by certain peptide derivatives of asparagine, and these are less active on a molar basis than is L-asparagine; L-aspartic acid, D-asparagine, and L...
متن کاملStudies on the asparagine requirement of the Jensen sarcoma and the derivation of its nutritional variant.
When Jensen sarcoma cells were cultured in medium devoid of free L-asparagine, the cell population decreased exponen tially for 10 days. The surviving cells then began an exponen ti@alproliferation phase. The resulting population did not quire asparagine to maintain proliferation. Population growth curves and cloning studies showed that, per 106 original cells, approximately 102 variant cells w...
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ژورنال
عنوان ژورنال: Journal of Cellular Physiology
سال: 1971
ISSN: 0021-9541,1097-4652
DOI: 10.1002/jcp.1040770317